RNA

Part:BBa_K3425038:Design

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-17)


Qβ5' recognition sequence of Qβ replicase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 61
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 64
    Illegal XhoI site found at 68
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 61
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 61
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is not BioBrick compatible due to the presence of XbaI site, however note that the site overlaps with dam methylation site GATC which blocks most XbaI enzymes. BBa K3425038 was assembled further using the Type IIS iGEM standard assembly. You can find more information about the cloning of this part as well as similar parts, which do not fit into the four predefined categories in Type IIS standard (promoter, RBS, CDS, terminator) in the Type IIS guidebookof Uppsala 2020 team.

Source

Synthesized according to
[1] Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.

References

[1]
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078